产品说明
特异性
Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.
免疫原
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.
Epitope: cytoplasmic domain, C-terminus
应用
Research Category
Cell Structure
ELISA/RIA: 1:1000.
Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.
Immunohistochemistry: 1:500
Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.
Optimal working dilutions must be determined by end user.
Research Sub Category
Integrins
Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.
联系
Replaces: 04-1131
外形
Liquid rabbit antiserum with 0.05% sodium azide.
储存及稳定性
Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | chicken, rat, pig, human, mouse, hamster |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable radioimmunoassay: suitable western blot: suitable |
NCBI登记号 | NM_000885.4 |
UniProt登记号 | P13612 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |