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Sodium 2-(4-hexylphenyl)diazene-1-sulfonate

品牌
Sigma-Aldrich
CAS
158896-12-9
货号
919233
规格纯度
(0.5% solution in 25mM aqueous ammonium bicarbonate, pH 7.0), ≥95%
参考价格
2462.19 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

4-Hexylphenylazosulfonate (Azo) is a photocleavable anionic surfactant developed by the Ge lab for use in high-throughput top-down and bottom-up proteomics by mass spectrometry (MS). Initially, the reagent showed a breakthrough as an improved method for top-down analysis [Brown et al. 2019]. Top-down analysis looks at intact proteins, while bottom-up analysis uses digested proteins. Of the two, top-down analysis is preferable for detecting post-translational modifications and sequence variations. Bottom-up is more commonly used and is likely to detect more proteins. This reagent was also shown to work for sample extraction that can be used for both top-down and bottom-up analysis on the same sample to maximize data collection[Brown et al. 2020].

Surfactants are required for protein extraction especially for challenging classes of protein including membrane proteins[Brown et al. 2020] and extracellular matrix proteins[Knott et al. 2020], but surfactants suppress the signal in MS. While removing a surfactant will improve the signal, this can cause protein loss and degradation. Azo allows for easy removal since it can be rapidly degraded using ultraviolet irradiation (λmax = 305 nm). Using Azo in this way reduces protein loss and gives an improved signal. Please see the Ge lab publications for information on using this product [Brown et al. 2019, Brown et al. 2020, Knott et al. 2020, and Aballo et al. 2021].

法律信息

PCT/US2019/035447

基本信息

经验(实验)分子式C12H17N2O3S · Na
分子量292.33

产品性质

质量水平100
测定≥95%
形式liquid
折射率n/D 1.334
密度0.994 g/mL
储存温度2-8℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

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