产品说明
一般描述
SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.
应用
SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.
特点和优势
- 采用随机引物技术扩增片段化DNA，如ChIP或FFPE
- 有助于对低至100 pg的ChIP DNA进行测序
- 采用增强型引物，可获得完整的基因组覆盖、最低的序列偏差、引物清除以及适用于二代测序的扩增子大小
- 可兼容用于二代测序的Illumina®、SOLiD™或454文库制备
其他说明
SEQXE-500RXN 是按需生产。获取更多信息，请发邮件至techserv@sial.com 联系技术服务。
法律信息
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of sigma-aldrich Co. LLC
iCAT is a registered trademark of University of Washington
基本信息
| NACRES | NA.55 |
产品性质
| 质量水平 | 200 |
| technique(s) | whole genome amplification: suitable |
| 运输 | wet ice |
| 储存温度 | −20℃ |
组份列表
组份可单独销售
| 货号 | 组份 |
| W4502 | Water, Nuclease-Free Water, for Molecular Biology |
| Library Preparation Buffer |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
