产品说明
一般描述
This PhosphoDetect
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
免疫原
a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
Human
包装
100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Immunoblotting (1:1000)
ELISA (1:2000)
警告
Toxicity: Standard Handling (A)
外形
In PBS, 1 mg/ml BSA, 50% glycerol.
重悬
Do not aliquot.
分析说明
Positive Control
RhoA GST fusion protein
其他说明
Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.
Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 同位素/亚型 | IgG |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
