PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

产品说明

一般描述

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

免疫原

KLH conjugated to phosphotyrosine, glycine, and alanine

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (1:2000)

Immunoblotting (1:500-1:2000)

Immunohistochemistry (1:500)

警告

Toxicity: Standard Handling (A)

外形

In PBS, 50% glycerol, 1% BSA, pH 7.4.

其他说明

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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安全信息