产品说明
一般描述
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
This PhosphoDetect
免疫原
KLH conjugated to phosphotyrosine, glycine, and alanine
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
警告
Toxicity: Standard Handling (A)
外形
In 10 mM sodium phosphate, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Any cell lysate or tissue extract
其他说明
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | PY20, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG2b |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
