产品说明
一般描述
Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.
特异性
Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.
免疫原
purified recombinant mouse TNF-α.
应用
Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunoprecipitation
- neutralization
包装
200 μL in glass insert
生化/生理作用
TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.
外形
Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01632994 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | hamster |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | TN3-19.12, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | rat, mouse |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~2 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable neutralization: suitable western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α |
| UniProt登记号 | P16599 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Tnf(21926) rat ... Tnf(24835) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
