产品说明
一般描述
Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.
Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.
特异性
This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 ℃.
应用
The enzyme functions optimally between 30-37 ℃ and suffers from degradation when subjected to temperatures above 50 ℃. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 ℃.
包装
1mg/ml in Plastic
制备说明
Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 ℃.
重悬
Reconstitute in sterile water.
储存及稳定性
Store product at -20℃. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.
其他说明
For R&D only.
基本信息
| 酶学委员会(EC)编号 | 3.4.21.50 ( BRENDA | IUBMB ) |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli (206-473aa) |
| 形式 | lyophilized powder |
| 浓度 | 1 mg/mL |
| 最佳pH | 8.5 |
| pH范围 | 9.0 - 9.5 (catalytic) |
| UniProt登记号 | P15636 |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H315 - H317 - H319 - H334 |
| 预防措施声明 | P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
