产品说明
一般描述
Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.
Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.
特异性
This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 ℃.
应用
The enzyme functions optimally between 30-37 ℃ and suffers from degradation when subjected to temperatures above 50 ℃. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 ℃.
包装
1mg/ml in Plastic
制备说明
Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 ℃.
储存及稳定性
Store product at -20℃ for up to one year. Avoid freeze/thaw cycles.
其他说明
For R&D only.
基本信息
酶学委员会(EC)编号 | 3.4.21.50 ( BRENDA | IUBMB ) |
产品性质
质量水平 | 200 |
生物来源 | Achromobacter lyticus |
重组 | expressed in E. coli (206-473aa) |
形式 | buffered aqueous glycerol solution |
浓度 | 1 mg/mL |
最佳pH | 8.5 |
pH范围 | 9.0 - 9.5 (catalytic) |
UniProt登记号 | P15636 |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |