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Lactic Dehydrogenase, recombinant from E. coli

乳酸

品牌
Sigma-Aldrich
CAS
9001-60-9
货号
59747
规格纯度
≥90 U/mg
参考价格
3202.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

应用

Lactic Dehydrogenase, recombinant from E. coli has been used:

  • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
  • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
  • in in vitro DltC D-alanylation assay

生化/生理作用

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
L -乳酸脱氢酶催化 L -乳酸转化为 L -丙酮酸,同时还原 NAD + 为 NADH 和 H +

单位定义

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25℃ (NADH as cofactor)

基本信息

酶学委员会(EC)编号1.1.1.27 ( BRENDA | IUBMB )
EC 号232-617-8
MDL编号MFCD00131466
NACRESNA.54

产品性质

质量水平100
重组expressed in E. coli
形式powder
specific activity≥90 U/mg
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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