产品说明
一般描述
LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.
应用
Lactic Dehydrogenase, recombinant from E. coli has been used:
- in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
- to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
- in in vitro DltC
D -alanylation assay
生化/生理作用
Conversion of
单位定义
One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25℃ (NADH as cofactor)
基本信息
酶学委员会(EC)编号 | 1.1.1.27 ( BRENDA | IUBMB ) |
EC 号 | 232-617-8 |
MDL编号 | MFCD00131466 |
NACRES | NA.54 |
产品性质
质量水平 | 100 |
重组 | expressed in E. coli |
形式 | powder |
specific activity | ≥90 U/mg |
储存温度 | −20℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |