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Imprint® Ultra Chromatin Optimization Kit

品牌
Sigma-Aldrich
货号
CHROP
规格纯度
Kit designed to optimize sonication parameters for ChIP experiments
参考价格
4388.92 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.

组分

Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.

法律信息

Imprint is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.54

产品性质

质量水平200

组份列表

组份可单独销售

货号组份
I8896IGEPAL® CA-630, for molecular biology
S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
W4502Water, Nuclease-Free Water, for Molecular Biology
A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

安全信息

象形图GHS02GHS03GHS05GHS07GHS09
警示用语:Danger
危险声明H226 - H271 - H290 - H302 - H314 - H336 - H410
预防措施声明P210 - P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310
危险分类Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Skin Corr. 1B - STOT SE 3
靶器官Central nervous system
储存分类代码5.1A - Strongly oxidizing hazardous materials
WGKWGK 3
闪点(F)100.4 °F
闪点(C)38 ℃

Sigma-Aldrich

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