产品说明
一般描述
Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
Note: 1 mU = 1 milliunit.
包装
200 miu in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
外形
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
其他说明
Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 3.2.1.96 ( BRENDA | IUBMB ) |
产品性质
| 质量水平 | 100 |
| 重组 | expressed in E. coli |
| 形式 | liquid |
| specific activity | ≥3 units/mL ≥30 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | do not freeze |
| 异质活性 | N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
