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Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)

品牌
Sigma-Aldrich
货号
GR37
规格纯度
liquid, clone 9A7γE10.4, Calbiochem®
参考价格
6526.19 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).
Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.

免疫原

purified, chick intestine vitamin D receptor

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1:50-1:100)

Gel Shift (see comments)

Immunoblotting (1:200-1:400)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)

警告

Toxicity: Standard Handling (A)

分析说明

Positive Control
Breast carcinoma tissue (see comments)

其他说明

Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源rat
抗体形式purified antibody
antibody product typeprimary antibodies
克隆9A7γE10.4, monoclonal
形式liquid
包含≤0.1% sodium azide as preservative
species reactivitychicken, rat, porcine, mouse, amphibian, human, hamster
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG1
运输wet ice
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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