产品说明

一般描述

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.

免疫原

unpurified polyubiquitinylated lysozyme
human

包装

100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

应用

ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

制备说明

Raji cells

重悬

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

其他说明

Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息