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Anti-p300 Mouse mAb (NM-11)

品牌
Sigma-Aldrich
货号
NA46
规格纯度
liquid, clone NM-11, Calbiochem®
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

免疫原

Epitope: within amino acids 2071-2091 near the CBP C-terminus
recombinant, human p300 protein
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 50% glycerol.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
A431, HeLa, or ML-1 cells

其他说明

Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.
Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆NM-11, monoclonal
形式liquid
不包含 preservative
species reactivityhuman, rat
should not react with mouse
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG1
运输wet ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

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