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Anti-Ly49A Antibody, clone A1

品牌
Sigma-Aldrich
货号
MABF2090
规格纯度
clone A1, from mouse
参考价格
1415.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

T-cell surface glycoprotein YE1/48 (UniProt: P20937; also known as Lymphocyte antigen 49a, Ly-49a, T lymphocyte antigen A1) is encoded by the Klra1 (also known as Ly-49, Ly-49a, Ly49, Ly49A) gene in murine species. LY49A is a single-pass type II membrane protein that serves as an inhibitory receptor on natural killer (NK) cells for H-2d(Dd) MHC class I molecules on target cells. It inhibits the activity of NK cells thereby preventing cell lysis. Ly49A-mediated NK cell inhibition is shown to be dependent on a lateral binding site, which is located beneath the peptide-binding platform of Dd. Ly49A is reported to contribute to target cell adhesion and accumulate at synapses with Dd-expressing target cells when NK cells themselves lack Dd. In cases where NK cells display Dd expression Ly49A does not contribute to adhesion. Higher expression of LY49A is observed in T-lymphoma cell lines and normal lymphocytes exhibit low expression. LY49A has a cytoplasmic domain (aa 1-44), a transmembrane domain (aa 45-66), and an extracellular domain (aa 67-262). (Ref.: Back, J., et al. (2007). Proc. Natl. Acad. Sci. USA 104(10); 3978 3983).

特异性

Clone A1 specifically detects Lymphocyte antigen 49a (LY49A) in murine species.

免疫原

EL-4 T lymphoma cells.

应用

Immunoprecipitation Analysis: A representative lot detected Ly49A in Immunoprecipitation applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86).

Immunocytochemistry Analysis: A representative lot detected Ly49A in Immunocytochemistry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83).

Flow Cytometry Analysis: A representative lot detected Ly49A in Flow Cytometry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83; Silver, E.T., et. al. (2000). J Immunol. 165(4):1771-81).

This mouse monoclonal Anti-Ly49A, clone A1, Cat. No. MABF2090 is tested for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation, for the detection of Lymphocyte antigen 49a.

质量

Evaluated by Flow Cytometry in EL-4 T lymphoma cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Ly49A in 1X10E6 EL-4 T lymphoma cells.

目标描述

30.50 kDa calculated.

外形

Protein G purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆A1, monoclonal
species reactivitymouse
包装antibody small pack of 25 μg
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
同位素/亚型IgG2aκ
UniProt登记号P20937

Sigma-Aldrich

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