Anti-LDL Receptor Mouse mAb (15C8)

产品说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

免疫原

Bovine
a trypic digest of bovine low density lipoprotein receptor
Epitope: first cysteine-rich ligand binding domain

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (0.5-1 g/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 g/ml)
Immunoblotting (1-5 g/ml, see application references)
Immunocytochemistry (1-5 g/ml, IF)
Immunoprecipitation (1-2 g/sample)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

分析说明

Positive Control
Liver sections, HT1080 cells, HepG2 cells

其他说明

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺² with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443.
Russell, D.W., et al. 1984. Cell37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664.
Goldstein, J.L., et al. 1979. Nature279, 679.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息