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Anti-EWS Antibody, clone 21B1

品牌
Sigma-Aldrich
货号
MABE1932
规格纯度
clone 21B1, from rat
参考价格
1415.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RNA-binding protein EWS (UniProt: Q01844; also known as EWS oncogene, Ewing sarcoma breakpoint region 1 protein) is encoded by the EWSR1 (also known as EWS) gene (Gene ID: 2130) in human. EWS is member of the TET family of DNA and RNA-binding proteins, which also includes translocated in liposarcoma/fused in sarcoma protein (FUS/TLS) and TATA-binding protein-associated factor 15. It is a multifaceted RNA binding protein with established roles in transcription, pre-mRNA processing, and DNA damage response. It contains a transcriptional-activation domain (EAD), 3 glycine-arginine rich regions, an RNA-binding domain, and a zinc finger domain (aa 518-549). EWS is highly methylated on arginine residues and methylation is mediated by PRMT1 and to some extent by PRMT8. Various environmental signals are known to induce post-translational modifications in its RNA binding domain and glycine-arginine rich domains, thus modulating EWS activity. EWS can relocate from cytoplasm to ribosomes upon PTK2B/FAK2 activation. Mutations in EWS gene are known to cause Ewing sarcoma, a highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue.

特异性

Clone 21B1 is a rat monoclonal antibody that detects human RNA-binding protein EWS. It targets an epitope with in 20 amino acids from the C-terminal region.

免疫原

Ovalbumin-conjugated linear peptide corresponding to 20 amino acids from the RGG3 domain of human RNA-binding protein EWS.

应用

Anti-EWS, clone 21B1, Cat. No. MABE1932, is a highly specific rat monoclonal antibody that targets RNA-binding protein EWS and has been tested in ELISA and Western Blotting.
ELISA Analysis: A representative lot detected EWS in ELISA applications (Suarez-Calvet, M, et. al. (2016). Acta Neropathol. 131(4):587-604).

Western Blotting Analysis: A representative lot detected EWS in Western Blotting applications (Suarez-Calvet, M, et. al. (2016). Acta Neropathol. 131(4):587-604).

Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EWS in HeLa cell lysate.

目标描述

~85 kDa observed; 68.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆21B1, monoclonal
species reactivityhuman
包装antibody small pack of 25 μL
technique(s)ELISA: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_053733
UniProt登记号Q01844

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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