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Anti-EF5 Antibody, clone ELK3-51 Antibody, Cyanine 5 conjugate

品牌
Sigma-Aldrich
货号
EF5012
规格纯度
clone ELK3-51, from mouse, CY5 conjugate
参考价格
7470.43 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

A number of imaging markers which target hypoxia exist and are widely used in research. EF5 (2-(2-Nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide) is a compound developed at the University of Pennsylvania by Dr. Cameron Koch and Dr. Sydney Evans. Upon injection into animal tissues, EF5 selectively binds to hypoxic cells and forms adducts. A mouse monoclonal antibody, clone ELK3-51, which is directly conjugated to Cyanine 5, is then used to selectively bind the EF5 adducts providing a sensitive and quantitative method to detect and measure tissue hypoxia in animal and human tumors, normal tissues and cells.

Advantages of the EF5 hypoxia detection method:
• EF5 exists in only one form; Pimidozole, an alternative hypoxia marker, exists in two forms; one of which is charged and very hydrophilic, the other lipophilic. Pimidozole thus has a very complex biodistribution. In contrast, EF5 is lipophilic and uncharged and this allows very rapid and even tissue distribution.
• EF5 binding images can be calibrated to provide quantitative data on the pO2 values of each cell (1). The fluorescent images obtained from EF5 binding can be calibrated according to camera settings and a “cube-binding” value which is obtained through a separate procedure. The intensity values of calibrated images are directly related to actual tissue pO2 values. As a result, these images provide information regarding not only where hypoxic areas may or may not be, but also data regarding the distribution and levels of hypoxia.

Reference:
Koch CJ (2002) Measurement of absolute oxygen levels in cells and tissues using oxygen sensors and 2-nitroimidazole EF5. Methods in Enzymology 352: 3-31.

应用

Anti-EF5 Antibody, clone ELK3-51 Antibody, Cyanine 5 conjugate is provided in a concentrated format at 2 mg/mL. For optimal performance, antibody should be diluted to a working concentration of at least 75 ug/mL. Refer to product datasheet for details.
Research Category
Cancer
Research Sub Category
Hypoxia
The EF5 hypoxia detection method provides a sensitive and quantitative method to detect and measure tissue hypoxia in animal and human tumors, normal tissues and cells.

储存及稳定性

Store at -20°C.

其他说明

Concentration: Please refer to product datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源mouse
偶联物CY5 conjugate
antibody product typeprimary antibodies
克隆ELK3-51, monoclonal
species reactivitymouse, rat, human
technique(s)immunofluorescence: suitable
immunohistochemistry: suitable
同位素/亚型IgG1

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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