位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Chikungunya virus Antibody, clone 3E7b

品牌
Sigma-Aldrich
货号
MABF2052
规格纯度
clone 3E7b, from mouse
参考价格
1415.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Chikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. This monoclonal antibody (3E7b) can bind strongly to native CHIKV surface and potently neutralize virus replication (Ref.: Marsinoul, P., et al. (2014). Virology 464-465; 111-117; Lam, S., et al. (2015). mAbs 7(6), 1178-1194).

特异性

Clone 3E7b detects Chikungunya virus in cells infected with this virus.

免疫原

Chikungunya virus

应用

Western Blotting Analysis: A representative lot detected Chikungunya virus in Western Blotting applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

Immunocytochemistry Analysis: A representative lot detected Chikungunya virus in Immunocytochemistry applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Chikungunya virus in BHK cells infected with Chikungunya virus. (Courtesy from an independent lab).

Neutralizing Analysis: A representative lot neutralized Chikungunya virus in Neutralizing applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

ELISA Analysis: A representative lot detected Chikungunya virus in ELISA applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

Anti-Chikungunya virus, clone 3E7b, Cat. No. MABF2052, is a mouse monoclonal antibody that detects E2 protein of Chikungunya virus and has been tested for use in ELISA, Immunocytochemistry, Neutralizing asssay, and Western Blotting.
Research Category
Inflammation & Immunology

质量

Isotype testing: Identity Confirmation by Isotyping Test.

Isotype Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

外形

Format: Purified
Purified mouse monoclonal antibody IgM in PBS without azide.

Purified by ion-exchange chromatography

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆3E7b, monoclonal
species reactivityvirus
包装antibody small pack of 25 μL
technique(s)ELISA: suitable
immunocytochemistry: suitable
neutralization: suitable
western blot: suitable
同位素/亚型IgMκ

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号