产品说明
一般描述
Recognizes the ~70-75 kDa AFP protein in HepG2 cells.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
免疫原
A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
包装
100 μg in Glass bottle
应用
ELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
警告
Toxicity: Regulatory Review (Z)
外形
In PBS, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
HepG2 cells
Negative Control
293T cells
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1G7, monoclonal |
| 形式 | liquid |
| 不包含 | preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
