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Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis-Calbiochem

品牌
Sigma-Aldrich
货号
176908
包装型号
规格纯度
参考价格
5551.43 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Recombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
Recombinant, Bacillus anthracis anthrax protective antigen(nicked) expressed in a special strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.

包装

100 μg in Plastic ampoule

生化/生理作用

Product does not compete with ATP.
Primary Target
EF and/or LF
Reversible: no
Cell permeable: no

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.

重悬

Following reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
Reconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.

分析说明

Single major band at 63 kD by SDS-PAGE

其他说明

Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7045.
Nassi, S., et al. 2002. Biochemistry41, 1445.
Singh, Y., et al. 1999. Infect. Immun.67, 1853.
Leppla, S.H. 1988. Methods Enzymol.165, 103.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
形式lyophilized
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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