Anti-CD18 Mouse mAb (IB4)

产品说明

一般描述

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.

免疫原

Human
human peripheral blood monocytes

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (5-10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Cell Inhibition Assays (see comments)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

重悬

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

分析说明

Negative Control
Nalm-6 cells
Positive Control
HPB-MLT cells

其他说明

Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息