产品说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant human YSK4 (MAP3K19) (941-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat (1). The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring (2) in the large-conductance Ca2+-activated K+ channels (3). The YSK4 kinase function has not been made clearly yet.
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 30-45 nmol/min·mg (radiometric) |
| 分子量 | ~83 kDa |
| 浓度 | 0.05 μg/μL |
| 登记号 | NM_025052 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... YSK4(80122) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
