产品说明
应用
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE). Applied voltages of less than 5 V/cm are recommended for maximum resolution.
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性(7M 尿素)凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离)。
包装
Foil pouches
制备说明
采用经生物技术性能认证的 Trizma 碱(产品代码 T6066)、分子生物学试剂硼酸(产品代码 B6768)和 EDTA 二钠盐(产品代码 E5134)制备。
重悬
Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 89mM Tris-Borate, 2mM EDTA, pH 8.3 at 25 ℃. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. Recommended temperature for dissolution 5 ℃ to 40 ℃.
储存及稳定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
法律信息
pHast Pack is a trademark of sigma-aldrich Co. LLC
基本信息
| MDL编号 | MFCD00236358 |
| NACRES | NA.21 |
产品性质
| 产品线 | pHast Pack™ |
| 形式 | powder |
| pH值(酸碱度) | 8.3 |
| SMILES string | OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O |
| InChI | 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H |
| InChI key | OSBLTNPMIGYQGY-UHFFFAOYSA-N |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H360FD |
| 预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| 危险分类 | Repr. 1B |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
