产品说明
一般描述
Tau-441 human, recombinant, is an isoform of Tau, variant 2N4R, having 4 microtubule binding repeats (R) and 2 amino terminal inserts (N). The uniprot identifier is P10636-8 (isoform Tau-F).It is expressed in HEK 293 cells, and is glycosylated.
生化/生理作用
Tau-441 is a member of the Tau family of proteins. Tau is a family of neuronal microtubule-associated proteins. Six isoforms have been found that differ from each other in having either 3 or 4 binding repeats (R) of 31-32 amino acids, and from zero to 3 amino terminal inserts (N) of 29 amino acids each.
Tau proteins are mainly expressed in the neurons of the central nervous system where they exert a role in stabilizing microtubules, key components of axonal transport, as well as in signal transduction. Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules. Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared
misfolded proteins, such as beta-amyloid precursor protein (beta-APP), ubiquitin, and various chaperones and protein kinases, are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport
system. Among other diseases, Tau forms in cerebrospinal fluid are considered a reliable biomarker for progressive supranuclear palsy, where the levels of Tau forms ratio were significantly reduced.
序列
MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGLDRIRGRKLGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ
This sequence includes a polyhistidine and V5 tags at the C-terminus.
外形
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
基本信息
| NACRES | NA.32 |
产品性质
| 重组 | expressed in HEK 293 cells |
| 质量水平 | 200 |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | calculated mol wt 50.3 kDa (including c-terminal His and V5 tags) observed mol wt 70-100 kDa by SDS-PAGE (The protein migrates as a 70-110 kDa protein on SDS-PAGE due to glycosylation) |
| UniProt登记号 | P10636-8 |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
