Sorbitol Assay Kit

产品说明

一般描述

Sorbitol (glucitol) is a sugar alcohol that is metabolized slowly in the human body. Sorbitol can be obtained from glucose by reducing an aldehyde group to a hydroxyl group. Accumulation of excessive sorbitol in erythrocytes, retinal cells, and Schwann cells has been associated with retinopathy, cataracts, peripheral neuropathy and diabetes. Sorbitol is made solely from corn syrup, and found in fruits such as apples, pears, peaches, and prunes. It is widely used as a sugar substitute and as a laxative. It is also utilized in specialty culture media and in healthcare, food and cosmetic products. Sorbitol is measured in biological samples to monitor metabolic pathways and the progression of diabetes.

特点和优势

• Linear detection range (20 μL sample): 5 to 1000 μM D-sorbitol.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism in biological (e.g. blood), food, beverage and agriculture samples.

原理

The sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum at A565 nm. The increase in absorbance at A₅₆₅ nm is directly proportional to the sorbitol concentration.

产品性质

安全信息