产品说明
一般描述
The Src homology region 2 domain-containing phosphatase-1 (SHP1)/protein tyrosine phosphatase, non-receptor type 6 (PTPN6) is a 68 kDa protein present in the cytoplasm. It is mapped to human chromosome 12p13.
Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, MW = 95 kDa, expressed in an E. coli expression system.
应用
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
生化/生理作用
In mammary epithelial cells, protein tyrosine phosphatase, non-receptor type 6 (PTPN6) controls EGFR (epidermal growth factor receptor) signaling. It participates in the JAK/STAT (janus kinase/signal transducers and activators of transcription) signaling pathway. PTPN6 regulates cytokine receptors like interleukin- 3 (IL-3) and erythropoietin.
单位定义
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 95 kDa |
| 包装 | pkg of 20 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | 1 mg/mL |
| NCBI登记号 | NM_080549 |
| UniProt登记号 | P29350 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PTPN6(5777) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
