位置:首页 > 品牌 > Sigma-Aldrich >

RNA Polymerase II, C-terminal human

品牌
Sigma-Aldrich
货号
SRP2013
规格纯度
recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
参考价格
5537.66 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RNA polymerase II (eukaryotic) contains around 10 subunits with a combined molecular weight of 500,000Da. The subunit structure of this enzyme is conserved amongst eukaryotes, and this subunits structure shares similarities with that of RNA pol I and III. The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms.

生化/生理作用

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo, designated IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongating complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

产品性质

生物来源human
重组expressed in E. coli
测定≥85% (SDS-PAGE)
形式frozen liquid
分子量 ~42.2 kDa
包装pkg of 10 μg
储存条件avoid repeated freeze/thaw cycles
浓度400 μg/mL
颜色clear colorless
NCBI登记号NM_000937
UniProt登记号P24928
运输dry ice
储存温度−70℃
Gene Informationhuman ... POLR2A(5430)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号