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Pyruvate kinase M2 Active from mouse

品牌
Sigma-Aldrich
货号
SRP0189
规格纯度
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
参考价格
9157.71 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Pyruvate kinase M2 (PKM2), also known as pyruvate kinase 3 (PK3), is an isoform of pyruvate kinase that is expressed in some differentiated tissues, such as lung, fat, retina, and pancreatic islets, as well as in normal proliferating cells and embryonic cells. PKM2/pk3 is encoded by the gene mapped to mouse chromosome 9.
Murine Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_011099), amino acids 2-end, with N-terminal His tag, MW = 58kDa, expressed in an Escherichia coli expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

Elevated expression of PKM2 is observed in most of the cancer cells. Hence, this protein can be considered as a potent therapeutic target for cancers. PKM2 plays an important role in the discharge of excessive intracellular reactive oxygen species (ROS), which otherwise damage cellular components and decrease cell viability. Protein kinase activity of PKM2 is involved in the regulation of gene expression and tumorigenesis. PKM2 is an important rate-limiting enzyme involved in final step of glycolysis and it has an essential role in metabolic reprogramming throughout cancer progression.

单位定义

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37℃

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源mouse
重组expressed in E. coli
测定≥90% (SDS-PAGE)
形式aqueous solution
分子量 58 kDa
包装pkg of 20 μg
储存条件avoid repeated freeze/thaw cycles
浓度>0.02 mg/mL
NCBI登记号NM_011099
UniProt登记号P52480
运输dry ice
储存温度−70℃
Gene Informationmouse ... PKM2(18746)

Sigma-Aldrich

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