产品说明
一般描述
Protein arginine methyltransferase 5 (PRMT5) and WD repeat-containing protein (WDR77) or methylosome protein- 50 (MEP-50) are part of the methylosome. PRMT5 is a type II PRMT enzyme. The gene encoding it is localized to human chromosome 14q11.2.
Complex of human PRMT5 (GenBank Accession No. NM_006109), amino acid 2-end, with N-terminal FLAG®-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), amino acid 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recombinant PRMT5/MEP50 has been used in the detection of arginine methylation in in vitro methyltransferase assay.
生化/生理作用
Methylosome protein 50 (MEP50) binds to the oncogenic protein arginine methyltransferase 5 (PRMT5) and forms a complex called methylosome. This complex is involved in mono- and symmetric dimethylation of arginine, which is necessary for various biological processes. MEP50 acts as a coactivator for androgen receptor (AR) and estrogen receptor (ER). PRMT5 also methylates histones and other proteins involved in genome organization, transcription, stem cells, primordial germ cells, differentiation, the cell cycle, and spliceosome assembly. The two proteins have been associated with cancer tumorigenesis and are marked as potential oncogenes.
单位定义
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37℃.
外形
Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 μg/ml FLAG® peptide, and 20% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
法律信息
FLAG is a registered trademark of sigma-aldrich Co. LLC
FreeStyle is a trademark of Invitrogen Corp.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in FreeStyle™ 293-F cells |
| 测定 | ≥60% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 37.5 kDa (MEP50) 73 kDa (PRMT5) |
| 包装 | pkg of 20 μg |
| manufacturer/tradename | sigma-aldrich |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_006109 (PRMT5) NM_024102 (MEP50) |
| UniProt登记号 | O14744 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PRMT5(10419) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
