产品说明
一般描述
Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A405 nm on a spectrophotometer.
特点和优势
- No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.
原理
The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 ℃.
产品性质
| 质量水平 | 100 |
| detection method | colorimetric |
| 储存温度 | −20℃ |
安全信息
| 象形图 |     |
| 警示用语： | Danger |
| 危险声明 | H302 + H332 - H314 - H341 - H373 - H411 |
| 预防措施声明 | P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
