产品说明
一般描述
Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | aqueous solution |
| specific activity | ≥1.1 pmol/min-μg |
| 分子量 | 146 kDa (p110δ) 83.5 kDa (p85α) |
| 包装 | pkg of 20 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_005026 (p110delta) XM_043865 (p85alpha) |
| UniProt登记号 | O00329 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PIK3CD(5293) |
