PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

产品说明

一般描述

This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser¹⁵.


Recognizes the ~53 kDa p53 protein phosphorylated at Ser¹⁵ in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.

免疫原

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH

包装

50 μL in Plastic ampoule

应用

Immunoblotting (1:1000)
Immunoprecipitation (1:200)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

分析说明

Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents

其他说明

Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息