产品说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant human NEK8 (1-322) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK8 or NIMA-related kinase 8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans which may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development (1). NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins (2).
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 5.0-7.0 nmol/min·mg |
| 分子量 | ~66 kDa |
| 浓度 | 0.05 μg/μL |
| 登记号 | NM_178170 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... NEK8(284086) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
