产品说明
一般描述
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.
特点和优势
- Detection limit of 0.02 μM and linearity up to 1 μM NAD+/NADH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 minutes.
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for the determination of NAD and NADH and the evaluation of drug effects on NAD/NADH metabolism in cells, tissue extracts etc.
原理
The NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
产品性质
| 质量水平 | 100 |
| application(s) | pharmaceutical |
| detection method | fluorometric |
| 相关疾病 | cancer, Alzheimer′s disease; neurological disorders, Parkinson′s disease; aging/geriatric diseases |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
