产品说明

应用

Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),⁽⁸⁾ and also from other bacteria that are difficult to lyse with lysozyme only.
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。

生化/生理作用

变溶菌素是一种 N-乙酰胞壁质酶。如溶菌酶一样，它是一种可裂解细菌细胞壁聚合物肽聚糖多糖的 β-N-乙酰胞壁酰-(1→4)-N-乙酰葡糖胺键的胞壁质水解酶。其羧基末端部分与识别和结合唯一的细胞壁聚合物有关。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。

单位定义

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 ℃ in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

外形

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

法律信息

Ficoll is a registered trademark of Cytiva

基本信息

产品性质

安全信息