产品说明
应用
MULTI-seq, multiplexing using lipid-tagged indices, is a novel tool that facilitates multiplex single-cell and single-nucleus RNA sequencing1. This technology provides sample barcoding that is compatible with any cell type or nucleus containing an accessible plasma membrane and allows for streamlined, pooled single-cell sample processing.
特点和优势
- Efficient. MULTI-seq increases the throughput and decreases the reagent cost of droplet-based single-cell RNA sequencing.
- Flexible. Compatible with any droplet generation platform, the indexing system enables users to run and analyze up to 96 barcoded samples simultaneously in an 8-lane droplet device.
- Clearer Results. The indices provide data quality advantages over non-indexed analysis methods in the form of doublet identification and retention of data from cells with low RNA content.
组分
The MULTI-seq Lipid Modified Oligos kit is comprised of a lignoceric amide-modified anchor DNA oligo solution and a palmitic amide-modified co-anchor DNA oligo solution. Together, these lipid-modified oligos embed into cell or nuclei membranes and provide a landing pad for DNA barcodes with a complementary 5′ sequence. The MULTI-seq Lipid Modified Oligos kit is intended for upstream sample preparation only, before pooling and single cell analysis.
NOTE: Unique barcode oligos are not included and must be purchased separately.
Barcode 3′ oligo design with poly-A tail for mRNA enrichment1:
5′-CCTTGGCACCCGAGAATTCCA-8-base index-A30-3′
Barcode oligo design for 5′ cDNA library synthesis:
5′-CCTTGGCACCCGAGAATTCCA-8-base index-CCCATATAAGAAA-3′
Custom barcode oligos may be ordered from SigmaAldrich.com/nextgenoligos. For minimal cross-contamination of barcode primers, order Next-Gen Sequencing Oligos with NGSO-Silver or NGSO-Gold quality.
Reagents Provided
- LMO001A Lignoceric Anchor with DNA Oligo
- LMO001B Palmitic Co-anchor with DNA Oligo
产品性质
| 质量水平 | 200 |
| 形式 | liquid |
| 用途 | sufficient for 100 sample(s) (100RXN kit is adequate for labelling 100 samples when using 1 μL of each LMO per sample) sufficient for 30 sample(s) (30RXN kit is adequate for labelling 30 samples when using 1 μL of each LMO per sample) |
| 浓度 | 50 μM (each) |
| technique(s) | multiplexing: suitable (Single Cell and Single Nucleus) |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
