产品说明
一般描述
This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human MTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.
应用
Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.
生化/生理作用
Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. MTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory associated protein of MTOR complex 1 (RAPTOR) has an important role in modulating cell growth and in energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.
基本信息
NACRES | NA.32 |
产品性质
生物来源 | human |
重组 | expressed in baculovirus infected Sf9 cells |
测定 | ≥50% (SDS-PAGE) |
形式 | aqueous solution |
分子量 | 137 kDa (mTOR) 150 kDa (Raptor) 37 kDa (MLST8) |
包装 | pkg of 10 μg |
NCBI登记号 | NM_004958 (mTOR) NM_020761 (Raptor) NM_022372 (MLST8) |
运输 | dry ice |
储存温度 | −70℃ |
Gene Information | human ... MLST8(64223), MTOR(2475), RPTOR(57521) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |