产品说明
一般描述
Monoclonal Anti-Sirt1 (mouse IgG2b isotype) is derived from the hybridoma SIRT1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Sirt1 recombinant protein. Sirtuin 1 is a member of mammalian Sir2 gene family. It is present in the nucleus.
免疫原
human Sirt1 recombinant protein
应用
Monoclonal Anti-SIRT1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting, immunoprecipitation and flow-cytometry.
生化/生理作用
Sirtuin 1 (Sirt1) is an NAD-dependent deacetylase and regulates various transcription factors, such as p53, FOX (forkhead box), nuclear factorΚB, myocyte enhancer factor-2 (Mef2) and peroxisome proliferator-activated receptor γ coactivator 1 α (PGC1α). Sirt1 deacetylates and downregulates p53. Increased activity of Sirt1 in culture cells reduces p53 transcriptional activity and p53-mediated apoptosis in response to radiation or oxidative stress. In addition, it also deacetylates and represses the activity of the forkhead transcription factor FOXO3a, other mammalian forkhead factors and reduces forkhead dependent apoptosis. Sirt1 plays a key role in myocyte differentiation by binding to the myoD cofactor, pCAF. It has been shown that Sirt1 modifies chromatin and silences transcription of integrated reporter genes via histone deacetylation. Sirt1 provides protection against neurodegeneration in models for Alzheimer′s disease and amyotrophic lateral sclerosis.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | SIRT1-1, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | human |
| 浓度 | ~1 mg/mL |
| technique(s) | flow cytometry: 5-10 μg/test using HeLa cells. immunoblotting: 1-2 μg/mL using whole extracts of human Jurkat cells. immunoprecipitation (IP): 5-10 μg using extracts of human Jurkat cells |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | Q96EB6 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... Sirt1(23411) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
