产品说明
一般描述
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.
免疫原
Recombinant full-length human DNA Ligase III-b.
应用
Suggested starting dilutions are as follows: Blocking: Assay-dependent dilution, ICC/IF: Assay-dependent dilution, IP: Assay-dependent dilution, WB: Assay-dependent dilution, PLA: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
生化/生理作用
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.
特点和优势
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
外形
Phosphate-buffered saline, no preservative added.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 1F3, monoclonal |
| 形式 | liquid |
| 分子量 | 100 kDa |
| species reactivity | mouse, chicken, human |
| 浓度 | 1 mg/mL |
| technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable proximity ligation assay: suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P49916 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... LIG3(3980) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
