产品说明
一般描述
Monoclonal Anti-GRAMD4 (mouse IgG1 isotype) is derived from the hybridoma GR4-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. GRAM domain containing 4 (GRAMD4) is localized in the nucleus and is mapped to human chromosome 22q13.31.
特异性
Monoclonal Anti-GRAMD4 recognizes human and monkey GRAMD4.
免疫原
synthetic peptide corresponding to a sequence at the N-terminal region of human GRAMD4
应用
Monoclonal Anti-GRAMD4 antibody produced in mouse has been used in:
- immunoblotting
- immunocytochemistry
- flow cytometry
生化/生理作用
GRAM domain containing 4 (GRAMD4) also known as DIP (Death-inducing protein), is a novel p53- independent pro-apoptotic target of E2F Transcription Factor 1 (E2F1), which localizes to the nucleus. Upregulation of GRAMD4 by E2F1 mediates apoptosis in karyotypically normal fibroblasts and tumor cells. GRAMD4 translocation to the mitochondria is mediated by p73- induced apoptosis. It interacts with B-cell lymphoma 2 (Bcl-2), promotes BCL2 associated X, apoptosis regulator (Bax) mitochondrial relocalization and oligomerization. It induces mitochondrial membrane permeabilization mediating the release of cytochrome c and second mitochondria-derived activator of caspases (Smac). Interestingly, GRAMD4 overexpression inhibits tumor growth. Its efficacy was more pronounced over time when combined with cisplatin, leading to prolonged growth retardation.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For extended storage, freeze at -20 ℃in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | GR4-1, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~80 kDa |
| species reactivity | monkey, human |
| 浓度 | ~1 mg/mL |
| technique(s) | flow cytometry: 2.5-5.0 μg/test using using HeLa cells. immunoblotting: 0.5-1 μg/mL using using whole extracts of Jurkat cells. immunocytochemistry: suitable immunofluorescence: 2-4 μg/mL using using HeLa cells. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q6IC98 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... GRAMD4(23151) mouse ... Gramd4(223752) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
