产品说明
一般描述
Monoclonal Anti-FUS (mouse IgM isotype) is derived from the hybridoma FUS-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FUS, conjugated to KLH. Fused in sarcoma (FUS), also known as translocated in liposarcoma (TLS), ribonucleoprotein (RNP)-P2, amyotrophic lateral sclerosis-6 (ALS6), is a RNA/DNA binding protein. FUS is normally located predominantly in the nucleus whereas, pathological FUS inclusions are mostly found in the cytosol of neurons and glia cells.
免疫原
synthetic peptide corresponding to an internal sequence of human FUS, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 3 amino acids in mouse and rat.
应用
Monoclonal Anti-FUS antibody produced in mouse has been used in various immunochemical techniques including:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化/生理作用
Fused in sarcoma (FUS) plays a vital role in transcription, RNA splicing and transport and is implicated in multiple diseases. Mutations in TAR DNA binding protein 43 (TDP-43) and FUS is associated with the development of amyotrophic lateral sclerosis (ALS) and fronto-temporal lobar degeneration (FLTD) including ubiquitin-positive inclusions (FLTDU). The majority of the FUS mutations has been recognized in C-terminal nuclear localization signal (NLS). FUS has been implicated in a broadening spectrum of neurodegenerative disorders. FUS has been identified as a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1-3).
外形
Culture supernatant solution containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | tissue culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | FUS-4, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~70 kDa |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: 1:500-1:1000 using formalin-fixed and paraffin embedded rat cerebellum. indirect immunofluorescence: 1:200-1:400 using using HeLa or HepG2 cells. western blot: 1:1000-1:2000 using lysates of G361 cells. |
| 同位素/亚型 | IgM |
| UniProt登记号 | P35637 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... FUS(2521) mouse ... Fus(233908) rat ... Fus(317385) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
