产品说明
一般描述
Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.
特异性
Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1
应用
Monoclonal Anti-FMR1 antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- immunofluorescence
- flow cytometry
生化/生理作用
Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For extended storage, freeze at 20 ℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | FMR2.5, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~71 kDa |
| species reactivity | hamster, human, rat, mouse |
| 浓度 | ~1 mg/mL |
| technique(s) | flow cytometry: 2.0-5.0 μg/test using HeLa cells immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts. immunocytochemistry: suitable immunofluorescence: 5-10 μg/mL using HepG2 cells. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q06787 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... FMR1(2332) mouse ... Fmr1(14265) rat ... Fmr1(24948) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
