产品说明
一般描述
Monoclonal Anti-EEF1A1 (mouse IgG2b isotype) is derived from the hybridoma EF48 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Eukaryotic elongation factor 1 A-1 (EEF1A1), also known as EF-l α is a GTP-binding protein. EEF1A1 is one of the most abundant cytoplasmic proteins and is ubiquitously expressed except in neurons and muscle.
Eukaryotic translation elongation factor 1 α 1 (EEF1A1) is a cellular protein which is part of the eukaryotic elongation factor 1 (eEF1) complex. The gene encoding it is localized on human chromosome 6q14.1.
免疫原
a synthetic peptide corresponding to an internal sequence of human EEF1A1
应用
Monoclonal Anti-EEF1A1 antibody produced in mouse has been used in immunoblotting and immunofluorescence.
生化/生理作用
Eukaryotic elongation factor 1 A-1 (EEF1A1) is involved in proper codon-anticodon binding interactions between mRNAs and tRNAs at the tRNA acceptor site of the ribosome. EEF1A also plays a translation-independent role in embryogenesis, senescence, oncogenic transformation, cell proliferation, apoptosis, cytoskeletal organization and protein degradation. Overexpression of EEF1A1 has been observed in various tumors of the pancreas, colon, breast, lung, and stomach.
Eukaryotic translation elongation factor 1 α 1 (EEF1A1) binds to aminoacetylated tRNAs and delivers them to the elongating ribosome during protein synthesis. It aids in the association of the eukaryotic elongation factor 1 (eEF1) complex with the HIV-1 (human immunodeficiency virus-1) reverse transcription complex. EEF1A1 also associates with genomic RNA secondary structures of RNA viruses and helps in their replication.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
质量水平 | 200 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | EF48 |
形式 | buffered aqueous solution |
species reactivity | bovine, rat, mouse, canine, monkey, human |
浓度 | ~1 mg/mL |
technique(s) | immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells. immunofluorescence: 0.5-1 μg/mL using SW-620 cells. |
同位素/亚型 | IgG2b |
UniProt登记号 | P68104 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... EEF1A1(1915) mouse ... Eef1a1(13627) rat ... Eef1a1(171361) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |