产品说明
一般描述
Monoclonal Anti-Dynamin 2 (DNM2) (mouse IgG1 isotype) is derived from the hybridoma DYN2-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Dynamin 2 (DNM2) also known as DYN2 belongs to the dynamins family of guanosine triphosphatase (GTPase) proteins.
The gene DNM2 (dynamin 2) is mapped to human chromosome 19p13. It is widely expressed. The protein has a catalytic amino-terminal GTPase domain, a middle domain, a pleckstrin homology domain (PH), a GTPase effector domain (GED) and a less conserved carboxyl-terminal proline/arginine rich domain (PRD).
免疫原
synthetic peptide corresponding to a sequence at the N-terminal region of human DNM2 , conjugated to KLH
应用
Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
Furthermore, DNM2 is upregulated in pancreatic cancer as it activates Ras-related C3 botulinum toxin substrate 1 (Rac1). It also promotes invasive cellular migration and lamellipod protrusion.
DNM2 (dynamin 2) is a GTPase which is required for clathrin-mediated endocytosis. It is important for clathrin-coated pit maturation and clathrin-coated vesicle formation. DNM2 participates in neuronal morphology, axonal growth, centrosome cohesion, actin- and microtubular organization. Mutations in it are associated with Charcot-Marie-Tooth disease, centronuclear myopathy ADCNM (autosomal dominant centronuclear myopathy) and lethal congenital contractures syndrome type 5 (LCCS5). It is upregulated in prostate cancer.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | DYN2-11 |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~98 kDa |
| species reactivity | monkey, canine, rat, mouse, human |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 2-4 μg/mL using using whole extract of HeLa cells. immunofluorescence: 5-10 μg/mL using using HeLa cells. immunoprecipitation (IP): 5-10 μg using using whole extract of HeLa cells. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P50570 |
| 储存温度 | −20℃ |
| Gene Information | human ... DNM2(1785) mouse ... Dnm2(13430) rat ... Dnm2(25751) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
