产品说明
一般描述
Monoclonal Anti-diMethyl-Histone H3 (diMe-Lys9) (H3K9me2) (mouse IgG1 isotype) is derived from the hybridoma 5E5-G5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a dimethylated (diMe-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH. Histones H3 and H4 are the core histones forming nucleosome, which is the fundamental unit of chromatin. H3 and H4 remodified by methylation and are highly methylated in mammalian cells.
免疫原
dimethylated (diMe-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
应用
Monoclonal Anti-diMethyl-Histone H3 (diMe-Lys9) (H3K9me2) antibody produced in mouse may be used in several immunochemical techniques including immunoblotting (~17 kDa) and immunofluorescence.
生化/生理作用
Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination thought to play an important role in development and in cancer. Histone methylation, is a complex, dynamic process involving various biological processes including transcriptional regulation, chromatin condensation, mitosis and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. Methylation of H3 at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 5E5-G5, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~17 kDa |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoblotting: 2-4 μg/mL using histones isolated from human HeLa cells. immunofluorescence: 1-2 μg/mL using human HeLa cells. |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
