产品说明
一般描述
The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
应用
To see more application data, protocols, vector maps visit sigma.com/shrna.
MISSION® pLKO.1-puro TurboGFP™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.
法律信息
使用本产品须遵守一项或多项许可证协议。详情请参阅 http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of sigma-aldrich Co. LLC
TurboGFP is a trademark of Evrogen Co.
基本信息
| MDL编号 | MFCD07785395 |
| NACRES | NA.51 |
产品性质
| 质量水平 | 200 |
| 产品线 | MISSION® |
| 浓度 | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
