产品说明
一般描述
Lipopolysaccharide (LPS), also known as endotoxin, is present mainly in the cell wall of Gram-negative bacteria. It comprises three structural regions the O-side chain, a middle polysaccharide component, and Lipid A which contributes to the pyrogenic nature of LPS.
应用
LPS, E. Coli O111:B4 has been used for priming macrophages to upregulate genes necessary for NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome activation. It has also been used to stimulate Raw264.7 cells before pull-down assay.
生化/生理作用
Lipopolysaccharide (LPS), an effective immunostimulant induces inflammatory and immune responses by activating Toll-Like Receptor 4 (TLR4). It also contributes to osteoclastogenesis in mice. LPS serves as a permeability barrier around the cell surface to restrict the entry of toxic molecules such as antibiotics and bile salts. It acts as a pyrogenic agent and eventually induces septic shock in host cells.
质量
分析：<通过 Lowry 分析，蛋白质为3％。
外形
PBS 中的液体，pH 7.4。
内毒素活性：通过凝胶法，每毫克 LPS 不少于 500,000 内毒素单位；生色(Cambrex 分析)毒性分析测量结果表明，LPS 约为 10 EU/纳克。
储存及稳定性
将未稀释的等分试样在 2-8°C 下可保持最多 6 个月。
任何脂多糖或脂质 A 产品的安全处理应采用良好的实验室技术。
这需要遵守以下规范：
1.穿着适当的实验室服装，包括实验服、手套和安全眼镜。
2.请勿用嘴吸移液管、吞咽或接触开放性伤口。 彻底清洗与产品接触的身体任何部位。
3.该产品是热原的。 与任何注射装置一起操作时，要格外小心，避免意外自动注射。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.41 |
产品性质
| 生物来源 | Escherichia coli |
| 质量水平 | 100 |
| manufacturer/tradename | Chemicon® |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
