Hyaluronidase Inhibitor Screening Assay Kit

产品说明

一般描述

Hyaluronidases are a family of enzymes that catalyze the degradation of the glycosaminoglycan, hyaluronic acid. Hyaluronic acid is one of the major constituents of the extracellular matrix in organisms where it contributes to both cell proliferation and migration. The role of hyaluronidases in breaking down this key factor in cell proliferation makes them a possible target for cancer treatment. One hypothesis is that increased hyaluronidase may help prevent tumor invasion by breaking down the extracellular matrix needed for tumor expansion. Conversely, decreasing hyaluronidase activity might prevent metastasis by stopping cancer cells from escaping primary tumor masses. The study to determine hyaluronidases′ exact role in cancer pathology is still ongoing.

特点和优势

• Rapid, entire assay can be completed in 30 minutes.
• Simple procedure with an enzymatic reaction and addition of stop reagent. No wash or reagent transfer steps involved.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the high-throughput screening for evaluation of hyaluronidase inhibitors.

原理

The Hyaluronidase Inhibitor Screening Assay Kit uses a two step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at A600 nm is directly proportional to the hyaluronidase activity in the sample.

产品性质

安全信息